3-(N-Maleimidopropionyl)biocytin
CAS No. : 102849-12-7
| Size | Price | Stock |
|---|---|---|
| 1mg | $38 | In-stock |
| 5mg | $75 | In-stock |
| 10mg | $120 | In-stock |
| 25mg | $240 | In-stock |
| 50mg | $410 | In-stock |
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| Cat. No. : | HY-W020784 |
| M.Wt: | 523.60 |
| Formula: | C23H33N5O7S |
| Purity: | >98 % |
| Solubility: | DMSO : 12.5 mg/mL (ultrasonic;warming;heat to 60°C) |
3-(N-Maleimidopropionyl)biocytin is a cysteine-specific labeling reagent and non-membrane-permeable probe. 3-(N-Maleimidopropionyl)biocytin covalently modifies the exposed cysteine residues of yeast Tim44, conjugating the biotin moiety to the polypeptide chain. 3-(N-Maleimidopropionyl)biocytin determines the topology of membrane proteins by modifying the exposed cysteine residues on the outer side of the inner mitochondrial membrane[1].
In Vitro:3-(N-Maleimidopropionyl)biocytin (0.5 mM; 30 min at 4 °C) labels the single cysteine residue (Cys-369) of yeast Tim44 in intact mitochondria, demonstrating that the C-terminal domain of yeast Tim44 is exposed to the mitochondrial intermembrane space[1].
3-(N-Maleimidopropionyl)biocytin (0.5 mM; 30 min at 4 °C) does not label rat liver Tim44 in either intact or solubilized mitochondria, demonstrating that all cysteine residues of rat liver Tim44 are inaccessible to the reagent[1].
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