Acridine Orange (zinc chloride salt)
CAS No. : 10127-02-3
(Synonyms: Euchrysine 3RX)
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| Cat. No. : | HY-D0942 |
| M.Wt: | 438.10 |
| Formula: | C17H20Cl3N3Zn |
| Purity: | >98 % |
| Solubility: | DMSO : 100 mg/mL (ultrasonic) |
Acridine Orange (Euchrysine 3RX) zinc chloride salt is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange zinc chloride salt produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm)[1][2][3].
In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[4].
1. Stain cells with Acridine Orange zinc chloride salt (1 μM; 20 min; 37℃).
2. Wash cells with PBS.
3. Cells are observed by a confocal laser scanning microscopy (FV3000, Olympus).
In Vivo: Acridine Orange (0.1 mg/kg, i.v., dogs) zinc chloride salt shows no clinical signs of toxicity and no abnormalities were seen in the blood within 30 days[1].
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